| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057223 |
| Chromosome: | chromosome 9 |
| Location: | 1621279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398650 | (1 of 1) PTHR21737:SF4 - CACTIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTTCGGGCTGCGGCTGTGGTGAGCCGTTTGCAGCGGCATGACGCTCT |
| Internal bar code: | GTTCAGACGTGACTGAAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 14.2857 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCGTGTCTAGGTAGCG |
| Suggested primer 2: | CTGATCATTTTCAGCGCGGG |