Insertion junction: CLIP2.057231_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057231
Chromosome:chromosome 12
Location:5212397
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527150 (1 of 1) PF09234 - Domain of unknown function (DUF1963) (DUF1963) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAAAGGTAAAGGGACGCATCCTGAGAGACAGCGCGAAGCTGACTGTC
Internal bar code:GTCACTCTGGGGCAAGGCTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:6198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGTGGACTGGACTGGAG
Suggested primer 2:CCCATGTACCCAAACCACGA