Insertion junction: CLIP2.057238_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057238
Chromosome:chromosome 12
Location:6973689
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g561050 ELG18,ELG19 (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 18 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACCACAATACTTCAAAGGGGACTATCTGTACCTGTCGCGGTTCATGA
Internal bar code:ACCTAGTCTGTAGGGCATCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACCTCCCAGTCTCCTCA
Suggested primer 2:TGGGAGCGAAGTAAAGCCTG