Insertion junction: CLIP2.057242_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.057242
Chromosome:chromosome 14
Location:440938
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g610700 PHC72 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAAACGCTGTCAAACCTGTAGGTGCTCGGAGGCCGCTGGACGGCAGG
Internal bar code:TTGGTTTAACGTTCTGGCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGACATCTGCTCTGGACCTC
Suggested primer 2:CACCGCACATGCAATCACAA