| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057244 |
| Chromosome: | chromosome 16 |
| Location: | 4949403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686500 | (1 of 1) PF03767 - HAD superfamily, subfamily IIIB (Acid phosphatase) (Acid_phosphat_B) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAATGCGGTACCAGAAGAGGCTGTAACATTAGTTTGAGGACGAGGTCA |
| Internal bar code: | CCTTGTGAAGGGGTTATCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1107 |
| LEAP-Seq percent confirming: | 86.7647 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGTGACGGGCAAAGGTA |
| Suggested primer 2: | CGTTGTTGGGCATGTGTTGT |