| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057250 |
| Chromosome: | chromosome 2 |
| Location: | 8512180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141701 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACCCAATTGACGTCAGCGTGTACCGGTAGCCTTTATGCCATACCAAG |
| Internal bar code: | CTCCTTTTGTCATTGTACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2428 |
| LEAP-Seq percent confirming: | 59.6491 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACTCCAGATGCAGGTT |
| Suggested primer 2: | TGACATGACACGGTTGGAGG |