| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057251 |
| Chromosome: | chromosome 11 |
| Location: | 2257981 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095148 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACACATGCTGCTACGCTTTACGAGATATCCCATCGAGCAGGCACCTGA |
| Internal bar code: | TGCCGGCGGCCGACCAGGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2607 |
| LEAP-Seq percent confirming: | 66.0377 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGGAACCTCATCCTGCT |
| Suggested primer 2: | CACCAACTTCGTGTACCCGA |