Insertion junction: CLIP2.057254_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.057254
Chromosome:chromosome 2
Location:1472507
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g083800 RHM2,SNE5 (1 of 1) K12450 - UDP-glucose 4,6-dehydratase (RHM); dTDP-glucose 4%252C6-dehydratase 3'UTR
Cre02.g083850 (1 of 173) IPR029058 - Alpha/Beta hydrolase fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGTCCTCAGTCTGCACGCCATTCCGCACGCCACCGCAGCGCATGCAGT
Internal bar code:AGCTATTATGGCAATCGGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:230
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCCTACGGTCTACCACT
Suggested primer 2:TCGTCTAGGCATCAGGTCCA