| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057254 |
| Chromosome: | chromosome 2 |
| Location: | 5491917 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114400 | LIPA2,LAS2 | (1 of 1) PTHR10949//PTHR10949:SF12 - LIPOYL SYNTHASE // LIPOYL SYNTHASE, CHLOROPLASTIC; Lipoic acid synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTCAACTGGGTTGGTGGCCCGGGTGTCAGAGTCTCGCGCGATGGGGG |
| Internal bar code: | GGTGTCCTTTTCGGACGGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCGAACACGACGTTG |
| Suggested primer 2: | TCATTCACCTGGTCAGCACC |