| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057255 |
| Chromosome: | chromosome 12 |
| Location: | 256863 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485300 | CSV6 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCGTCTAGCAGCGGTGATGTGGCATACATGGATGGGCGCACGGCTG |
| Internal bar code: | ATTGTAGCCGAATAGAAACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4054 |
| LEAP-Seq percent confirming: | 71.7949 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAATTTTGGCCGTTTCCT |
| Suggested primer 2: | AGTAATGGCGGTGGTGGTG |