| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057255 |
| Chromosome: | chromosome 12 |
| Location: | 256864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485300 | CSV6 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACAGCCCCACTTACAGCTGCCGCGTTGTCCCAAGTTATATACACATCC |
| Internal bar code: | ACTAGCGTAGTAGATAGCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAATGGCGGTGGTGGTG |
| Suggested primer 2: | GGCAATTTTGGCCGTTTCCT |