| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057266 |
| Chromosome: | chromosome 3 |
| Location: | 3201487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGACCTAGGCCGCCGCACAGTCCCCGTCAGCACTCATCAATACACCG |
| Internal bar code: | AAGGTCCCAGCTATCAAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTTCCTCCAACTCA |
| Suggested primer 2: | AAGTGATGTCTCGGCACAGG |