Insertion junction: CLIP2.057298_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.057298
Chromosome:chromosome 14
Location:2099831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g621600 (1 of 1) PF15003 - HAUS augmin-like complex subunit 2 (HAUS2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGACATAGTTGCCTCTGAGCAAACTACTCGAAGCCCGACAGCTCCCGT
Internal bar code:TGTCATCGTCCATCCCCACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2109
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CACAATGGTGCGGAAGTTGG
Suggested primer 2:GCCTAACCCCTCTCTCTCCA