Insertion junction: CLIP2.057304_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.057304
Chromosome:chromosome 1
Location:3229772
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAATCGCTGGCGGGATTGCCATGGACGGAAAAGTGGAAAAGGCTCATG
Internal bar code:CGTGCGCAGGGCGCGTCGCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1843
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAACCAGCAAGCTCCAGG
Suggested primer 2:CGTCGAGGTGGCTAGCTTAG