| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057317 |
| Chromosome: | chromosome 13 |
| Location: | 4519574 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603050 | 5'UTR | ||
| Cre13.g603100 | GT90-48,GT90F48 | GT90 family protein 48; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGCAGGGTTCGGGTTCGCAGCACACGGTTTCGTGCCCCTAGGTTCC |
| Internal bar code: | ATGTTGCACGGGGATGCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGCCGCTTCACATAAG |
| Suggested primer 2: | CAGCGTTTGGAGGTTCAACG |