| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.057323 |
| Chromosome: | chromosome 7 |
| Location: | 133597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313100 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTAACCAGAAAGTGTTACTTAGGCGGCATCTGGTGGATGCCGCGCTA |
| Internal bar code: | GTGCGGGCGACGTTTGTTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGATGATGAAGTCCGTA |
| Suggested primer 2: | CTGGGCTGTAAGTGGGGATG |