| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057328 |
| Chromosome: | chromosome 11 |
| Location: | 4332958 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482626 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATTGAGGCGGGCGGAGGGGACTGAGGCGAGAGGGTTCTGAGGCAGGG |
| Internal bar code: | TTAGACCACTAACGGGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTAATCACCATGCGCCA |
| Suggested primer 2: | GTACTGCGACTATCCACGGG |