Insertion junction: CLIP2.057339_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.057339
Chromosome:chromosome 1
Location:3991964
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g026150 (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase 3'UTR
Cre01.g026200 PPR9 PentatricoPeptide Repeat protein 9; (1 of 6) PF01535 - PPR repeat (PPR) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACCAACCTGCCTTACAACTGTCCCATCGCACACTGCACTGCCACAAC
Internal bar code:GATTGTACTCTCCCAAATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:703
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACGCTTCTGGGTGAGTG
Suggested primer 2:AGCAGCTGAAACGGACATGA