| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057349 |
| Chromosome: | plastome |
| Location: | 101365 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802307 | psbF,2716994,ChreCp043 | photosystem II reaction center subunit VI; (1 of 1) K02708 - photosystem II cytochrome b559 subunit beta (psbF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATACCGTGGATAGCTAACCAACGAACTGTGAAAATAGGATAAACAA |
| Internal bar code: | GCTTCGATCGGTTGGGAGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCCCTATTAGCTCAAACC |
| Suggested primer 2: | CCAAAAGCTGCAGGCAGTTC |