Insertion junction: CLIP2.057369_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.057369
Chromosome:chromosome 5
Location:840882
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246100 (1 of 1) IPR019887 - Transcription regulator AsnC-type, C-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGGGGTCTGCCAGCTGGCGCTGGCGCCCTGCTAGGCTGCATACGTAGC
Internal bar code:TAATCGTCACCAAAGTGTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1530
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTTGCTTGTACGCTGGCA
Suggested primer 2:GCACCTACAGCTTTCCGTCT