| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057381 |
| Chromosome: | chromosome 1 |
| Location: | 2100240 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011750 | (1 of 1) PTHR31353:SF1 - PROTEIN TAG-322 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAATAAAAAACTAGTCCTGTGTCTACGTGCGTCTCCCTACCTTGTCAG |
| Internal bar code: | CTCGGGCTACATCTGCCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTGTCTCCTTGCTGCTT |
| Suggested primer 2: | TGCGCGTGTGTGTTTGTTAG |