Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.057393 |
Chromosome: | chromosome 1 |
Location: | 5769907 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g041351 | (1 of 1) PTHR15967:SF0 - E2F-ASSOCIATED PHOSPHOPROTEIN | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTGGTGACCGGTGATGGATTGCAGGTAGTGGGCGGCTCGTGGGTTCT |
Internal bar code: | TGGACTTTGCGGGACTCTTTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3275 |
LEAP-Seq percent confirming: | 68.9655 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTCTGGCCGAGGACTAT |
Suggested primer 2: | GGCGCAATATGTGACGTCAC |