Insertion junction: CLIP2.057394_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057394
Chromosome:chromosome 1
Location:2400481
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g013700 ASC1,VDAC1 (1 of 2) K15040 - voltage-dependent anion channel protein 2 (VDAC2); Voltage-dependent anion-selective channel protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGCGCTCACAGGTCCGACGCTCGCGTACAATGATCGAGGCACCATT
Internal bar code:ACTCTGTGTTCCCGCTACGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3380
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GACAAGCTGTCCGTCCTCAA
Suggested primer 2:CAACAGAAAACCAGCGTGGG