Insertion junction: CLIP2.057395_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.057395
Chromosome:chromosome 9
Location:110255
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386550 (1 of 1) K12492 - ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACACATGCACGTTATGGTGGCGACATTACAAAGAAGAGACAGCCCAA
Internal bar code:TGTCCGTGTTTCTGGCGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2197
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGTTGACAACTGTGCTG
Suggested primer 2:TTTACTCGCACGGGGTGTAC