Insertion junction: CLIP2.057395_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.057395
Chromosome:chromosome 9
Location:110265
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386550 (1 of 1) K12492 - ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTTTCGACCCCTAGCAAGGGCCGGTCCCGCCCGTTCGGCTCCCCCGT
Internal bar code:TGTCCGTGTTTCTGGCGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1957
LEAP-Seq percent confirming:41.5094
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACTCGCACGGGGTGTAC
Suggested primer 2:GGCTGTTGACAACTGTGCTG