| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057398 |
| Chromosome: | chromosome 4 |
| Location: | 2859276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224350 | MOT55 | Predicted protein; (1 of 22) PTHR10994 - RETICULON | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAGCACAAAATCTCAAGGACATATGCGTTGTGCCAGCAGTGTTTTC |
| Internal bar code: | TGGGCCGCGCTAGCATGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 19.2308 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTACTTCATGGCATGCG |
| Suggested primer 2: | CCCGATGTCCCAATGTCCAA |