| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057400 |
| Chromosome: | chromosome 12 |
| Location: | 7931433 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553050 | NUP53 | (1 of 1) K14313 - nuclear pore complex protein Nup53 (NUP35, NUP53); Nucleoporin 53 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGGAAGGCTGCCCCCGCAGCGTCCGGCCCCTCTGCCAGGCTCTCCA |
| Internal bar code: | AATCGTCAGTTTATACTACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACAGTCTCTGCCTTGC |
| Suggested primer 2: | GGAGTGTTGTGGCTCGAAGA |