| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057402 |
| Chromosome: | chromosome 17 |
| Location: | 5543935 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739500 | (1 of 13) PTHR13244//PTHR32523//PTHR32523:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | 5'UTR | |
| Cre17.g739550 | CPLD12 | Conserved in the Plant Lineage and Diatoms; (1 of 11) 2.7.4.3 - Adenylate kinase / Myokinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCACAGCAGTGCCCTCATCACTAGCCCAAACTGCTCCTCCCGTGTCA |
| Internal bar code: | TGGGGGTTCTTCTGTAGTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1268 |
| LEAP-Seq percent confirming: | 53.6585 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGGCTTTCATTTCGTTT |
| Suggested primer 2: | TCGCGAGCATCCAATCTCTC |