| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057409 |
| Chromosome: | chromosome 5 |
| Location: | 1921245 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236039 | 3'UTR | ||
| Cre05.g236050 | (1 of 2) IPR015097 - Lung surfactant protein D coiled-coil trimerisation | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTGCCCATACCAGCCTATCCTCAGCCCCCTCCGGTACCCGGCCTCCC |
| Internal bar code: | CACGCGAAGTTTTAGTCATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1882 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCAAACGTCACACACAG |
| Suggested primer 2: | GCAAGCGCCTTCTATTGAGC |