| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057411 |
| Chromosome: | chromosome 2 |
| Location: | 8473316 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGCAACCTGAGCAGGGGGGGGGGTACGATTGTACGAACTAAACCTCC |
| Internal bar code: | ACGGCGTGTTGCCCCTAAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1575 |
| LEAP-Seq percent confirming: | 61.25 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAGTTCAGGGTCGGTCG |
| Suggested primer 2: | CAGTAGGCGTGCAGTGATGA |