| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057419 |
| Chromosome: | chromosome 10 |
| Location: | 5478684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGAAGCTGGACTCTTGCCAGGACTCTTTCCAGGGTTATCGTCGGGC |
| Internal bar code: | CCTATCGTTGAACATTCTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5089 |
| LEAP-Seq percent confirming: | 70.6522 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACGATGTTGGAGTCCT |
| Suggested primer 2: | AGTCCATGCCCACATCAGTG |