| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057425 |
| Chromosome: | chromosome 1 |
| Location: | 139923 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTCGAGCCAGATCCGCACCCTGGAGGCGCAGCTGCAGTCGCTGCGC |
| Internal bar code: | CCTCACCGCTGCTTTTAATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACGTAAAACAGCAGCC |
| Suggested primer 2: | GATAGTCGGCTTACCTGGCC |