| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057426 |
| Chromosome: | chromosome 15 |
| Location: | 5247562 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734677 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGGGGTTGTGCTGCTGGCTGCACGGATGTAACGTGTGCTGAGGTGC |
| Internal bar code: | CGGGAGGCTGCGGCCGCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2914 |
| LEAP-Seq percent confirming: | 9.21053 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACGCTGCTTACACCAA |
| Suggested primer 2: | CGCCATACCTCATACGCACT |