| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057427 |
| Chromosome: | chromosome 2 |
| Location: | 8262394 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143450 | (1 of 2) PF13301 - Protein of unknown function (DUF4079) (DUF4079) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACCGTGAATCAATGGTGTCCACACGCACCCCTGAATGCTGACCCGAC |
| Internal bar code: | GAAGCTCATTGAGGCGTTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACTGGGTCAGGTGTCGG |
| Suggested primer 2: | CACGTGGTTAAGTGTCCGGA |