| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057447 |
| Chromosome: | chromosome 1 |
| Location: | 6710135 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047950 | IFT27,Rabl4,FAP156 | (1 of 1) K07934 - intraflagellar transport protein 27 homolog (IFT27, RAYL, RABL4); Intraflagellar transport protein 27 | 3'UTR |
| Cre01.g048000 | TPM1 | Tropomyosin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTTGTTCCTAACCCCCCCCCCCTTTCGGAAGCTTACACAATCGCTGG |
| Internal bar code: | GAATGTTGATTTAGCTGACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2988 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTACAGCAGCAGCAAG |
| Suggested primer 2: | CACCACCTTCTACCGCAACT |