Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.057452 |
Chromosome: | chromosome 2 |
Location: | 3534477 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g099200 | (1 of 1) K06100 - symplekin (SYMPK) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGAGGTGGTCAAGGTCGTGTGGGAAAGCGGCCCTCTGGTGAGGGCCT |
Internal bar code: | TGTTTCATTGAAATACTGGGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1529 |
LEAP-Seq percent confirming: | 67.4419 |
LEAP-Seq n confirming: | 29 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGAGGCAGTGGAATCACAC |
Suggested primer 2: | CCGTACACATGCATGCTTCG |