| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057455 |
| Chromosome: | chromosome 17 |
| Location: | 3740992 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726400 | (1 of 6) K04710 - ceramide synthetase (CERS) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGGAGTGGCCCCCGACAGGCACATGCGCTCCCTGCCGCAAGCATGA |
| Internal bar code: | TATGCTGAGCAGTCCAAGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2447 |
| LEAP-Seq percent confirming: | 91.8919 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGAGTGTGCAGTGAGCT |
| Suggested primer 2: | CTACCTACGCGGCGTTGTAT |