| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057456 |
| Chromosome: | chromosome 6 |
| Location: | 1212540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258400 | (1 of 1) PF08737 - Rgp1 (Rgp1) | 3'UTR | |
| Cre06.g258450 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGTCCCTGCTGCACCCCTGTGCACGTCACGAACGACAGGACCTTGAA |
| Internal bar code: | GCTGGATTGATTGTTCCTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 1.5873 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCTTCATTGATGCGGG |
| Suggested primer 2: | ATCACTTCCTTCAACGGCGT |