| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057462 |
| Chromosome: | chromosome 7 |
| Location: | 3841254 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338750 | 3'UTR | ||
| Cre07.g800823 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCTTCATGTGATCTAAGCTTTTAACGCATCATTGTCATCAATGCAAA |
| Internal bar code: | ATGCTTTCTCGCAAGTAGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4328 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCTATGATGAGGCTCTT |
| Suggested primer 2: | TTTGGCATGGGCATTCAAGC |