| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057467 |
| Chromosome: | chromosome 12 |
| Location: | 9263576 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCCGCCACCACCGTTGC |
| Internal bar code: | ATACTTGGTTATGTGCGTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 3.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGGCAGGTCTGGATTG |
| Suggested primer 2: | CGTACCGATGTCGCTGCTAT |