Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.057473 |
Chromosome: | chromosome 10 |
Location: | 917969 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g424150 | TF2A4 | (1 of 1) K03123 - transcription initiation factor TFIIA small subunit (TFIIA2, GTF2A2, TOA2); Transcription initiation factor IIA, gamma subunit | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAGTAACCAATACTGATACAGGCGGGGGCGCATGTATGCGTAAGTGAA |
Internal bar code: | AATGCGGAGCACAGGATGACGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1644 |
LEAP-Seq percent confirming: | 50.7042 |
LEAP-Seq n confirming: | 36 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAACATCCGCAAATGCCGC |
Suggested primer 2: | CCCCGTCCACACACAACATA |