| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057488 |
| Chromosome: | chromosome 11 |
| Location: | 135324 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467537 | PPR12 | PentatricoPeptide Repeat protein 12 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGAGTAAAGCTCCGGTTTGTCAGGCATCAGCCAGCTGCAAAAAACA |
| Internal bar code: | CACATGTCTAGGTGATACGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3126 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCTCCTCGTAGGATGC |
| Suggested primer 2: | CAGCGACCCAGTTGAGTTCT |