| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.057515 |
| Chromosome: | chromosome 17 |
| Location: | 4803839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733350 | 3'UTR | ||
| Cre17.g733400 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAGGGTTATGTACCGAATCTGCCGGATGTAGAAATACAGCGTTCCGC |
| Internal bar code: | TTTCCAAGGTCAGTCTTGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1713 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACATTCGACGCGTTGAGA |
| Suggested primer 2: | GGCCTGCACTTAACCCTGAT |