| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.057532 |
| Chromosome: | chromosome 17 |
| Location: | 5606666 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739900 | (1 of 1) IPR012337//IPR027417 - Ribonuclease H-like domain // P-loop containing nucleoside triphosphate hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGTTGATTTCACGCGTGCCTGAGGGGGAAATGCTTCGTTGTGGAACC |
| Internal bar code: | CCAGGGCAGGTCTTAAGAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2946 |
| LEAP-Seq percent confirming: | 82.5 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCGGTGGTCTGGCTAC |
| Suggested primer 2: | CAACACGTCAACACCTGCAG |