| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.057539 |
| Chromosome: | plastome |
| Location: | 12644 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802267 | ChreCp005,2716979,tufA | elongation factor 1-alpha; (1 of 1) PTHR23115//PTHR23115:SF146 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGGTGCTGGTGTTGTAACTAATATTGTTCAATAATTTCACATTTAAA |
| Internal bar code: | GGGCAACTGGGTCGTGCAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 53.5714 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCCGCGTTTTCTCCGTG |
| Suggested primer 2: | CAGGTCGTGGTACTGTTGCT |