Insertion junction: CLIP2.057548_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.057548
Chromosome:chromosome 9
Location:5765726
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCAACGCTTCCCAAGGGCTGACCCCCCGTTGCGCCGAACAGCGCTGA
Internal bar code:TACTGATGTAAATCTTGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:144
LEAP-Seq percent confirming:31.25
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGGTGGTGAGAAGCAT
Suggested primer 2:AAATCCCCCTCACCTGCATG