| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057551 |
| Chromosome: | chromosome 3 |
| Location: | 2117201 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156650 | (1 of 5) 2.7.11.7 - [Myosin heavy-chain] kinase / Myosin II heavy-chain kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCACGTGCCAGGCAGTCGTACACCGCCGCCGTACGGCTGCCGTACCCG |
| Internal bar code: | TCTAAGGTCGTCGGGGAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1154 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCAACTCACTCACAGTC |
| Suggested primer 2: | ATTAGCGAGATCTGCGTGGG |