| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.057554 |
| Chromosome: | chromosome 6 |
| Location: | 527758 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252300 | PDK1 | Putative pyruvate dehydrogenase kinase; (1 of 1) 2.7.11.4 - [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase / Branched-chain oxo acid dehydrogenase kinase (phosphorylating) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGCCGATGAAGGCCGGGCGCTTGTTGTTGATGTGCAGGTGCTGCTC |
| Internal bar code: | CTCACGACGTACCTCGGCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTAGTCATGCCCACCTGC |
| Suggested primer 2: | TTGGCATGTGCTGAAAACGG |