| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.057556 |
| Chromosome: | chromosome 10 |
| Location: | 3832094 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445850 | MAE14 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF); MATE efflux family protein | outside_mRNA |
| Cre10.g445900 | (1 of 1) PF00226//PF14901 - DnaJ domain (DnaJ) // Cleavage inducing molecular chaperone (Jiv90) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAGTTAGGTTATCGGCCTCCGCATCGGGCGACTCGGAAGCCCGCCGG |
| Internal bar code: | AGTGGGGCACGAGTCAATCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3031 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACGGTGAAGACCAGACAA |
| Suggested primer 2: | AACAGGCTCACACGACTCTG |