| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.057567 |
| Chromosome: | chromosome 10 |
| Location: | 3041009 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440900 | RRP43 | Rrp42-like exosome component; (1 of 1) K12586 - exosome complex component RRP43 (RRP43, EXOSC8, OIP2) | 3'UTR |
| Cre10.g440950 | FAP192 | (1 of 1) PTHR14465//PTHR14465:SF0 - IQ DOMAIN-CONTAINING PROTEIN H // IQ DOMAIN-CONTAINING PROTEIN H; Flagellar Associated Protein 192 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTATTCCAGTCTAGTACATGCCTGTGTATCCAGCATCCCCGTTCCAAC |
| Internal bar code: | GGGGGTTCCGTTCGAAGATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2591 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGCAGGGTCTGTACAA |
| Suggested primer 2: | AGGGCGTCATCTTCAACCTG |